Detecting plant diseases such as Xylella fastidiosa requires a different approach from insect monitoring. Instead of counting individuals, field technicians must identify symptoms and collect plant material for laboratory analysis.

Within CERBERUS, this process follows a strict protocol to ensure that samples are reliable and traceable.

Technicians walk through olive plots, carefully inspecting trees for visible symptoms. Sampling focuses on plant material that shows clear signs of disease, typically branches or shoots with mature leaves. Young shoots are avoided to ensure that the sample reflects established infection.

Each sample must belong to a single plant, ensuring accurate traceability. Before placing the material in a bag, branches and leaves are shaken to prevent transporting potential insect vectors along with the sample.

Samples are stored in sealed plastic bags, labelled with a unique code and kept under controlled temperatures between 4°C and 8°C. Maintaining freshness is essential, particularly during warmer periods, which is why transport is carried out using insulated containers.

This methodology ensures that samples arrive at the laboratory in optimal condition, supporting accurate diagnosis and reliable monitoring.

Field validation of these protocols is ongoing across the CERBERUS pilot sites.